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fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss fluorescence microscope
    Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+vert+a1+microscope/Inverted+microscope+Axio+Vert%2EA1/pm42883341-148-5-7
    Average 97 stars, based on 1195 article reviews
    fluorescence microscope - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: NooQ5 Supplementation Promotes Myogenic Differentiation in C2C12 Cells Under Chemical and Oxidative Stress Models.
    Article Snippet: After three additional PBS-T washes, nuclei were counterstained with DAPI (1:1000, Invitrogen). .. Coverslips were mounted using Fluoromount-G mounting medium (Invitrogen) and visualized under an Axio Vert.A1 microscope (Zeiss) equipped with a 40× objective lens (NA = 0.55). .. Micrographs were processed using ZEN software (Zeiss 3.12), and the fusion index was automatically quantified using the Cellpose (3.1+) deep-learning segmentation framework.

    Article Title: Nebivolol-induced bi-directional modulation of intracellular kinases contributes to the inhibition of triple-negative breast cancer.
    Article Snippet: Stress-induced β-adrenergic signaling is widely recognized as a detrimental factor in breast cancer progression, and β-blockers have been shown to exert beneficial effects on breast cancer patients in various clinical settings.. However, the molecular mechanisms underlying these beneficial effects remain incompletely understood, which hampers the clinical application of existing β-blockers and the development of more effective anti-tumor agents targeting β-adrenergic signaling.. In this study, we investigated the third-generation β-blocker nebivolol to elucidate how this drug alters the phenotype of triple-negative breast cancer (TNBC) cells by modulating intracellular signaling pathways.

    Article Title: Dietary CoQ10 Ameliorates Age‐Related Megakaryocyte Dysfunction via COPS3 ‐Mediated Activation of Autophagy
    Article Snippet: Colonies were stained with acetylcholinesterase (AchE, MBL) and counted. .. For proplatelet formation, megakaryocytes were plated on fibrinogen‐coated (100 μg/mL) culture dishes and incubated at 37°C for 3 h. Proplatelet formation was visualized using a Carl Zeiss Axio Vert.A1 microscope (200 × magnification). ..

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: For cell counting and size, cells were trypsinized, resuspended in DMEM supplemented with 10% FBS and analyzed by automatic cell counter Luna II (Logos Biosystems). .. Cell morphology images were taken in an Axio Vert.A1 Microscope (Zeiss). ..

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: .. Images were taken using an Axio Vert.A1 Microscope (Zeiss). ..

    Article Title: Extracellular Vesicles from Senescent Tumor Cells Are Necessary and Sufficient to Drive Paracrine Senescence
    Article Snippet: .. Images were taken with Axio Vert.A1 Microscope (Zeiss) and 10 images per condition were quantified. .. Cellular lysates were prepared in 1x RIPA buffer containing protease and phosphatase inhibitor cocktails (4693159001 and 4906845001, Sigma-Aldrich).

    Article Title: Chikungunya Virus Infection of Monocytes Generates a Persistent Macrophage Reservoir That Promotes IFN-π/IL-27–STAT1- and NF-κB–Driven Chronic Arthritis
    Article Snippet: Data were analyzed using FlowJo software (version 7.6.2), and debris and dead cells were excluded based on forward- and side-light properties. .. MDM and MDM-CHIKV cultures were visualized and photographed using an Axio Vert.A1 microscope (ZEISS, Oberkochen, Germany) equipped with a Ds-fi1 camera (Nikon, Tokyo, Japan) and adapted for transmitted-light in-phase contrast and epi-fluorescence microscopy. .. Images were processed and analyzed using IrfanView (version 4.69), ImageJ (version 1.8.0), and GIMP (version 2.10.30).

    Incubation:

    Article Title: Dietary CoQ10 Ameliorates Age‐Related Megakaryocyte Dysfunction via COPS3 ‐Mediated Activation of Autophagy
    Article Snippet: Colonies were stained with acetylcholinesterase (AchE, MBL) and counted. .. For proplatelet formation, megakaryocytes were plated on fibrinogen‐coated (100 μg/mL) culture dishes and incubated at 37°C for 3 h. Proplatelet formation was visualized using a Carl Zeiss Axio Vert.A1 microscope (200 × magnification). ..

    Staining:

    Article Title: Nebivolol-induced bi-directional modulation of intracellular kinases contributes to the inhibition of triple-negative breast cancer.
    Article Snippet: Stress-induced β-adrenergic signaling is widely recognized as a detrimental factor in breast cancer progression, and β-blockers have been shown to exert beneficial effects on breast cancer patients in various clinical settings.. However, the molecular mechanisms underlying these beneficial effects remain incompletely understood, which hampers the clinical application of existing β-blockers and the development of more effective anti-tumor agents targeting β-adrenergic signaling.. In this study, we investigated the third-generation β-blocker nebivolol to elucidate how this drug alters the phenotype of triple-negative breast cancer (TNBC) cells by modulating intracellular signaling pathways.

    Transmitted Light:

    Article Title: Chikungunya Virus Infection of Monocytes Generates a Persistent Macrophage Reservoir That Promotes IFN-π/IL-27–STAT1- and NF-κB–Driven Chronic Arthritis
    Article Snippet: Data were analyzed using FlowJo software (version 7.6.2), and debris and dead cells were excluded based on forward- and side-light properties. .. MDM and MDM-CHIKV cultures were visualized and photographed using an Axio Vert.A1 microscope (ZEISS, Oberkochen, Germany) equipped with a Ds-fi1 camera (Nikon, Tokyo, Japan) and adapted for transmitted-light in-phase contrast and epi-fluorescence microscopy. .. Images were processed and analyzed using IrfanView (version 4.69), ImageJ (version 1.8.0), and GIMP (version 2.10.30).



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    The two pediatric medulloblastoma cell lines ONS76 (p53 wild-type) and UW228-3 (p53-mut.) were exposed to 20 Gy γIR. After the indicated time periods, cells were stained for senescence-associated beta-galactosidase activity (manifested in blue) and documented microscopically in phase contrast ( A ) and brightfield ( B ) using a Zeiss <t>Axio</t> <t>Vert.A1</t> microscope. With increasing incubation times after irradiation, ONS76 display a strong blue staining and morphological changes, such as an increase in cell and nucleus size, reminiscing of cellular senescence, whereas UW228-3 cells detach from the bottom and die. All pictures were taken with the same 10× objective and the included 100 µm scale bar is representative for all micrographs. The pictures chosen are representative for at least three independent biological experiments. Please note that detached and dead cells are aspirated during the SA-beta-Galactosidase staining procedure and are therefore not present anymore, in contrast to Supplementary Fig. , in which the cells were photographed under culturing conditions.
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    The two pediatric medulloblastoma cell lines ONS76 (p53 wild-type) and UW228-3 (p53-mut.) were exposed to 20 Gy γIR. After the indicated time periods, cells were stained for senescence-associated beta-galactosidase activity (manifested in blue) and documented microscopically in phase contrast ( A ) and brightfield ( B ) using a Zeiss <t>Axio</t> <t>Vert.A1</t> microscope. With increasing incubation times after irradiation, ONS76 display a strong blue staining and morphological changes, such as an increase in cell and nucleus size, reminiscing of cellular senescence, whereas UW228-3 cells detach from the bottom and die. All pictures were taken with the same 10× objective and the included 100 µm scale bar is representative for all micrographs. The pictures chosen are representative for at least three independent biological experiments. Please note that detached and dead cells are aspirated during the SA-beta-Galactosidase staining procedure and are therefore not present anymore, in contrast to Supplementary Fig. , in which the cells were photographed under culturing conditions.
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    Image Search Results


    The two pediatric medulloblastoma cell lines ONS76 (p53 wild-type) and UW228-3 (p53-mut.) were exposed to 20 Gy γIR. After the indicated time periods, cells were stained for senescence-associated beta-galactosidase activity (manifested in blue) and documented microscopically in phase contrast ( A ) and brightfield ( B ) using a Zeiss Axio Vert.A1 microscope. With increasing incubation times after irradiation, ONS76 display a strong blue staining and morphological changes, such as an increase in cell and nucleus size, reminiscing of cellular senescence, whereas UW228-3 cells detach from the bottom and die. All pictures were taken with the same 10× objective and the included 100 µm scale bar is representative for all micrographs. The pictures chosen are representative for at least three independent biological experiments. Please note that detached and dead cells are aspirated during the SA-beta-Galactosidase staining procedure and are therefore not present anymore, in contrast to Supplementary Fig. , in which the cells were photographed under culturing conditions.

    Journal: Cell Death Discovery

    Article Title: p53 wild-type/low c-Myc-expressing ONS76 pediatric medulloblastoma cells become senescent after gamma-irradiation and can be eliminated by senolytic substances

    doi: 10.1038/s41420-026-03404-4

    Figure Lengend Snippet: The two pediatric medulloblastoma cell lines ONS76 (p53 wild-type) and UW228-3 (p53-mut.) were exposed to 20 Gy γIR. After the indicated time periods, cells were stained for senescence-associated beta-galactosidase activity (manifested in blue) and documented microscopically in phase contrast ( A ) and brightfield ( B ) using a Zeiss Axio Vert.A1 microscope. With increasing incubation times after irradiation, ONS76 display a strong blue staining and morphological changes, such as an increase in cell and nucleus size, reminiscing of cellular senescence, whereas UW228-3 cells detach from the bottom and die. All pictures were taken with the same 10× objective and the included 100 µm scale bar is representative for all micrographs. The pictures chosen are representative for at least three independent biological experiments. Please note that detached and dead cells are aspirated during the SA-beta-Galactosidase staining procedure and are therefore not present anymore, in contrast to Supplementary Fig. , in which the cells were photographed under culturing conditions.

    Article Snippet: Pictures were taken with 10× or 20× magnification as indicated using the Zeiss Axio Vert.A1 microscope and the associated AxioVision Software (Carl Zeiss MicroImaging GmbH, Göttingen, Germany).

    Techniques: Staining, Activity Assay, Phase Contrast, Brightfield, Microscopy, Incubation, Irradiation